PEGFP N2 PDF

3 Oct Description: pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher. Provide pEGFP-N2 vector/plasmid map, full length sequence, antibiotic resistance, size and other information. pEGFP-N2 encodes a red-shifted variant of wild-type GFP which has been optimized for brighter fluorescence and higher expression in mammalian cells.

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Efficient cleavage requires at least two copies pegfp n2 the NarI recognition sequence. Mapping the brain, one cell type at a time Learn about pioneering efforts to map the mammalian brain using single-cell transcriptomics. AcGFP1 is a monomer, which makes it a superior alternative for fusion applications. This product utilizes our pegffp Capturem technology in a spin column format with membrane-immobilized prgfp.

Measure NFkB activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal.

质粒载体:pEGFP-N2

Speed up your mass spec workflow Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. Efficient cleavage requires at least two copies of the RsrII recognition sequence. Efficient cleavage requires at least pegfp n2 copies of the PluTI recognition sequence.

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Sticky ends from different TthI sites may not be compatible. EGFP has been reported to form dimers Jain pehfp al. Sticky ends from different AccI sites may not be compatible.

Get Snap Gene Viewer. All pegfp n2 are the property of Takara Bio Inc. Oligomerization of green fluorescent protein in the secretory pathway of endocrine cells. Additional product, intellectual property, and restricted use information is available at takarabio.

Season one Season two Season three BioView blog. Fusions to the N terminus of EGFP retain the fluorescent properties of the native protein allowing the localization of the fusion protein in vivo. The 1-base overhangs pegffp by PflFI may be hard to ligate. Sequences flanking EGFP have been converted to a Kozak consensus translation initiation site 6 to further increase the translation efficiency in eukaryotic cells. Sticky ends from different PflFI sites may not be compatible.

pEGFP-N2 pEGFP N2载体质粒图谱、序列、价格、抗性、大小等基本信息_生物风载体

For full activity, add fresh DTT. The inserted gene should include the initiating ATG codon.

Measure NFkB activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal.

The 1-base overhangs produced by PflFI may be hard to ligate. Our mission is to develop high-quality innovative tools and services to accelerate discovery. Log in to enjoy additional benefits Why sign up for an account?

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Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies protein coverage comparable to or better than those obtained using in-solution trypsin digestion. Our brands Takara Clontech Cellartis. Partner with Takara Bio! Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover.

Efficient cleavage requires at least two copies of the NarI recognition sequence. Sticky ends from different SfiI sites may not be compatible.

PEGFP N2 DOWNLOAD

Sequences flanking EGFP have been converted to a Kozak consensus translation initiation site 6 to further increase the pegfp n2 efficiency in eukaryotic cells. Use with any Living Colors vector that contains a neomycin resistance cassette to create double-stable cell lines.

Vectors for expressing and visualizing a protein of interest fused to AcGFP1, including a prelinearized vector for simple, one-step In-Fusion cloning. Read our cookie policy. Fusions to the N terminus of EGFP retain the fluorescent properties of the native protein allowing the localization of the fusion protein in vivo.